Subscribe to the Bio-Rad channel: more information, visit http://www.bio-rad.com/CFXMaes. CFX Maestro Software User Guide Version 2.0. CFXMaestroSoftware UserGuide Version2.0. N CFX n Drivers n Firmware n QuickStart. Focus t25 free utorrent video. Feb 12, 2020 CFX Maestro Software utilizes the GeNorm algorithm, the details of which are described in Vandesompele’s study (Vandesompele et al. Briefly, the ratio of a pair of reference genes and all pairwise variations is calculated across all samples. The M value is the arithmetic mean of all pairwise variations.
- Fast, Friendly, Flexible
- Designed for the Way You Work
- Real-Time PCR plate format thermocycler
- Quick set up the run and monitor amplification traces in real-time on the integrated LCD touch screen
- 5-Channel Real-Time PCR detection system combinates advance optical technology with precise temperature control
- Provide sensitive and reliable detection of multiplex reaction
- Quick set up – easy installation and factory- calibrated optics
- Sample volumes 1-50 µl
- Optimize reaction in a single run – thermal gradient feature
Basic properties:
- Sample capacity: 96 wells
- Dimension (W x D x H): 330 mm x 460 mm x 360 mm
- Weight: 21kg
Temperature parameters:
- Range: 0-100 °C
- Accuracy: ±0.2
- Uniformity: ±0.4
- Maximum ramp rate: 5 °C/sec
- Average ramp rate: 3.3 °C/sec
- Heating and cooling method: Peltier
- Lid: Heat up to 105 °C
- Operation range of gradient: 30-100 °C
- Programmable span: 1-24 °C
Optical Detection:
Maestro Nutrition Software
- Excitation: 6 filtered LEDs (1 for FRET analysis)
- Detection: 6 filtered photodiodes (1 for FRET analysis)
- Range of excitation/emission wavelengths: 450-730 nm
- Detects 1 copy of the target sequence in human genomic DNA
- Scan time: 12 s all channels, FAM/SYBR® Green only 3s
Channel Excitation Detection (nm) (nm) | Calibrated Fluorophores | |||||||
Green | 450-490 | 515-530 | FAMTM,SYBR Green ITM | |||||
Yellow | 515-535 | 560-580 | VIC®,HEXTM,TETTM,Cal Gold 540TM | |||||
Orange | 560-590 | 610-650 | ROXTM,TEXAS RED®,Cal Red 610TM | |||||
Red | 620-650 | 675-690 | CY5, Quasar 670TM | |||||
Crimson | 672-684 | 705-730 | Quasar 705TM | |||||
FRET | 450-490 | 560-580 | FRET Chemistry |
Cfx Maestro Software User Guide
Cfx Maestro software, free download Mac
Software:
- Operating system: Windows 10
- Multiplex analysis: Up to 5 targets per well
- Advanced data analysis tools: normalized gene expression using CFX Maestro Software with statistic analysis
- Run without a computer, run up to 4 cyclers from 1 computer